Figure 2. Serotonin from ADF Requires
ser-5 in AVJ to Elevate Pumping (A) Wild-type pumping rate of
tph-1 mutants in which
tph-1 is reconstituted in ADF alone is dependent on
ser-5 (
ser-5;
tph-1[Psrh-142::
tph-1]), but not on
ser-1 (
tph-1;
ser-1[Psrh-142::
tph-1]) or
ser-7 (
tph-1;
ser-7[Psrh-142::
tph-1]). (B)
ser-5 and
hlh-34 are required for elevation of pumping rate in response to 5 mM 5-HT. Reconstitution of
ser-5 within
hlh-34-expressing neurons, but not pharyngeal neurons (Pglr-7::
ser-5) or pharyngeal muscle (Pmyo-2::
ser-5), confers responsiveness to 5-HT. Genetic ablation of
hlh-34 neurons (Phlh-34::ICE) abrogates responsiveness to 5-HT. (C) Expression of Phlh-34::mcherry and Pser-5::
ser-5::gfp overlap in
hlh-34 neurons. Data are normalized mean ±SEM. For (A), ANOVA with Bonferroni correction for multiple comparisons. For (B), Student's t test. ***p < 0.001 versus well-fed animals, unless otherwise indicated. For (A) and (B), 2.5 kb upstream of the
hlh-34 start site was used to drive expression of mCherry. Images were taken with a spectral confocal microscope using a Z-stack and compressed into a single file. WT, wild-type. See also Figure S2.