Figure 4. Detection of endogenous PAR-4 protein on western blot. Protein extracts from
par-4(
it75) adult hermaphrodites, N2 (wild-type) adult hermaphrodites and N2 embryos were subject to SDS-PAGE, transferred to a nitrocellulose filter and probed with affinity-purified anti-PAR-4 antibody from rabbit 5-308. The same blot was re-probed with an anti-tubulin antibody as a loading control.