Fig 14. MADD-2 expression in the primordium. (A) Wild-type embryos expressing MADD-2::GFP (green) and the general membrane reporter (red) (n = 32embryos examined). The panels at right are high magnifications of the bracketed regions. (B) Diagram summarizing changes in MADD-2::GFP levels overtime, ranging from high (dark green) to low (white). Note that MADD-2::GFP disappears in both L and R cells in the
int1 ring after about 345 minutes, and remains detectable in both R and L cells in the
int8 and
int9 rings until at least 600 minutes. MADD-2::GFP expression was not detected in the dorsal midline valve cell,
v3D, which rotates counterclockwise (345 minutes), but was detectable in non-intestinal cells that move dorsally to flank
v3D (
pm8 at 345 minutes, see [9]). (C) MADD-2::GFP expression in an
apx-1(
zu347ts) mutant embryo following a shift to restrictive temperature (26 degrees C) at the E8 stage. MADD-2::GFP expression disappeared in the
int1 ring, as in wild-type embryos, but persisted in both R and L cells in the
int2-4 rings. The image is of the ventral side of the intestine; the
int2-
int4 rings did not rotate, so both R and L cells are visible at the ventral midline. Panels = A,B [JJ2536], C [JJ2537]. Bar = 5 microns.