Figure 1 Generation of IYR001 strain containing
sas-6(
luv1[
sas-6::ha]), and its localization in the early C. elegans embryo A) Left panel: Cartoon depicting nucleotide sequences flanking the ha-tag sequence in the designed repair template to generate the
sas-6::ha Red text- Silent mutations introduced in the repair template; Teal highlight- part of homology arms. Right panel: Cartoon depicting DNA fragment sizes expected upon PCR followed by NdeI digestion for wild-type and
sas-6::ha edited worms. B) Agarose gel electrophoresis of NdeI digested
sas-6 PCR products to screen for homozygous edited
sas-6(
luv1[
sas-6::ha]) worms. L: 1Kb DNA ladder, Yellow stars:
sas-6::ha homozygous worms. C) Immunostaining with an anti-HA antibody shows localization of SAS-6::HA to the centrosomes (insets) and in the cytoplasm in 1-cell C. elegans embryos during different stages of mitosis. Microtubules (red), SAS-6::HA (green) and DNA (blue).