Figure 5. UNC-95 is expressed in muscle attachment sites. (A) Expression of the transcriptional fusion reporter including 2.5 Kb of
unc-95 upstream sequences. Arrow points at one of the four body wall muscle quadrants. Bar, 50 um (B-E). Expression of the translational fusion UNC-95::GFP in developing embryos. The cytoplasmic expression is maintained through embryogenesis. Localization to the muscle attachment sites is shown in E. Arrow indicates one muscle quadrant. Arrowhead indicates the nucleus. Lateral view of the embryo is shown. Bars, 10 um. (F and G) Expression of the translational fusion UNC-95::GFP in the body wall muscles of adults. (F) UNC-95 is expressed in muscle dense bodies (arrowhead), in M-lines (thin arrow), and in muscle-muscle cell boundaries (thick arrow). (G) UNC-95 localization in the nucleus and cytoplasm of body wall muscles. Arrowhead indicates the nucleus. Expression is also detected in muscle arms (arrow). (H) UNC-95 is localized in the vulval muscles and is highly concentrated in the sites of muscle attachments to the hypodermis (two arrows indicate the strong GFP labeling in the region of attachment). (I) Localization to the anal depressor muscle (arrowhead). (J-L) Expression of the mutant protein
unc-95(
su33)::GFP translational fusion. (J) Weak staining could be detected in dense bodies and M-lines, but there is no staining in muscle-muscle cell boundaries. (K) Localization of the
unc-95(
su33)::GFP is maintained in nuclei (arrow). (L) Irregular pattern of staining of the
unc-95(
su33)::GFP translational fusion. Thin arrow indicates cell in which the transgene was silenced; arrowhead indicates cell in which the M-lines are much thicker; thick arrow indicates cell in which the dense bodies aggregate to abnormal structures. Bars, 10 um.