Figure 1.
sut-2 loss of function mutants suppress tau Tg lifespan and pharyngeal pumping phenotypes:A)
sut-2(
bk3011) and
sut-2(
bk3012) have a similar lifespan to N2 (wild-type control animals). B)
sut-2(
bk3011) and
sut-2(
bk3012) rescue the shortened lifespan of tau Tg animals. See Table 1 for lifespan numbers (N), mean, and p-values. C) Time-compressed recordings of C. elegans pumping activity over two minutes. Each line spike above and below the center describes the action potential of a single pump. tau Tg animals pump more often than N2, represented by a denser trace.
sut-2(
bk3011); tau Tg and
sut-2(
bk3012); tau Tg pumping is similar to that of N2. D) Expanded view of pharyngeal action potentials showing both positive (excitatory) and negative (relaxation) spikes, which delineate the beginning and end of a single action potential. The scale bar represents 200 ms of recording. E) tau Tg animals have significantly increased pump frequency compared to N2, p<0.0001.
sut-2(
bk3011); tau Tg, and
sut-2(
bk3012); tau Tg pump frequencies are not significantly different from N2. Average pump frequency over a 2 minute recording, N=17 for N2, N=22 for tau Tg, N=20 for
sut-2(
bk3011); tau Tg, and N=22 for
sut-2(
bk3012); tau Tg. Significance was evaluated using one-way analysis of variance with Tukey's multiple comparison test. F) tau Tg,
sut-2(
bk3011); tau Tg, and
sut-2(
bk3012); tau Tg animals have significantly decreased pump durations compared to N2, p<0.0001. Average pump duration over a 2 minute recording, N=17 for N2, N=22 for tau Tg, N=20 for
sut-2(
bk3011); tau Tg, and N=22 for
sut-2(
bk3012); tau Tg . Significance was evaluated using one-way analysis of variance with Tukey's multiple comparison test. G)
sut-2(
bk3011) and
sut-2(
bk3012) have similar pumping frequencies to N2. Average pharyngeal pumps per minute was assessed with manual counting, N=30 for N2, N=15 for
sut-2(
bk3011), and N=15 for
sut-2(
bk3012).