Fig. S7.Deletion of the
bed-3 upstream sequence affected
bed-3 expression in the SMcells and M descendants but not the vulva and the hypodermis. (A-D) Thedeletion did not affect
bed-3 expression in the vulva and the hypodermis. Reducedlevel of
bed-3 mRNA in the vulva of
sy705 mutants is likely due to nonsensemediated decay. Mid L4 animals were assayed. (E-H) The deletion reduced
bed-3expression in the SM lineage and M descendants. L3 animals were assayed forbed-3 TMR signal in the SM cells. Early L2 animals were assayed for
bed-3 TMRsignal in the M descendants. Each TMR spot represents a single
bed-3 mRNAmolecule. (B, D, F, H) Quantitation of
bed-3 mRNA level. Each circle/squarerepresents the number of
bed-3 mRNA molecules detected by smFISH in oneanimal. Unpaired t-test was used. Error bars represent SEM.