H3.3 Chromatin fractions extracted with NaCl
H3.3 NaCl Salt Extracted Chromatin (Henikoff project, Henikoff subgroup)
General DescriptionStaged C. elegans embryos were prepared from a worm strain engineered to express biotin-tagged histone H3.3. Chromatin isolated from these animals was digested with micrococcal nuclease, extracted with NaCl, and concentrated with streptavidin-coated beads. The bound fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.
Release Date: 2009-11-29