Embryo Fax-sorted cells TransFrags
embryo cells tiling arrays (Waterston project, Miller subgroup)
Cells were isolated using FACS, sorting on GFP-fusion reporters expressing embryos. RNA was isolated, amplified, and transcripts were detected on an Affymetrix C. elegans tiling array. A smoothed density plot (log 2 transformed with a window size of 110) and transcriptionally active regions (TARs) are shown.
Series DescriptionOur experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
Release Date: 2009-11-19