Adult_Mononucleosomes (Henikoff project, Henikoff subgroup)
General DescriptionStaged C. elegans adults were prepared from a worm strain engineered to express biotin-tagged histone H3.3 (note that the transgene was not induced during this particular experiment). Chromatin isolated from these animals was digested with micrococcal nuclease and extracted with 80 mM NaCl, followed by gel electrophoresis. The gel-purified fraction was used in two-color hybridization experiments with NimbleGen genomic tiling microarrays. The hybridization signal from this fraction was compared to the signal from the input DNA. Scaled log2(ratios) are shown.
Release Date: 2009-11-29