3RACE polyA-capture daf-2 dauer 454 alignments (Piano project, Kim subgroup)
3UTRs were isolated from dauer larva of C. elegans daf-2 worms using a poly-A capturing technique. RNAs were sequenced on the Roche 454 platform. This submission reflects the alignments of the deconvoluted, dauer stage-specific reads to WS190 genome using BLAT.
General DescriptionThe 3 untranslated region (3UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3RACE cloning-sequencing stragety to identify the 3UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
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Release Date: 2009-11-04