Core Histones ChIP-chip arrays

Core Histones (Lieb project, Ahringer subgroup)

General Description

Synchronized C. elegans early embryos from strain N2 were treated with the cross-linking reagent formaldehyde. Sonicated chromatin was prepared and immunoprecipitated with an affinity-purified polyclonal antibody that recognizes core histone H3. After whole genome amplification, NimbleGen genomic tiling microarrays were used in two-color hybridization experiments to compare the signal from the input DNA versus the fragments pulled-down in the ChIP. Normalized log2(ratios) are shown in this track.

Protocols

  1. Growth and isolation: Worm embryo growth and harvest:JL:SS6, Worm L3 extraction:JL:PK1, Worm embryo extraction:JL:SS2, Worm embryo growth and harvest:JL:SS3, Worm embryo growth and harvest:JL:SS4
  2. Sample preparation: Worm LM-PCR Amplification for ChIP-chip:JL:IL1, Worm Chromatin Immunoprecipitation:JL:SS2, ChIP-chip scanning nimblegen:JL:1, Worm Chromatin Immunoprecipitation:JL:PK1, ChIP-chip label hyb nimblegen:JL:1, Worm LM-PCR Amplification for ChIP-chip:JL:SS2, ChIP-chip scanning nimblegen:JL:2, Worm chromatin immunoprecipitation:JL:IL2, Worm Chromatin Immunoprecipitation:JL:SS4, ChIP-chip label hyb nimblegen:JL:2
  3. Other Protocols: ChIP-chip normalization standard nimblegen:JL:1, ChIP-chip normalization standard nimblegen:JL:1

Experimental Reagents

    Growth Conditions:
  1. Antibodies: C. elegans AR0144_H3 rabbit serum, C. elegans AB1791_H3 rabbit polyclonal antibody
  2. Arrays: 080922_MODENCODE_CE_CHIP_HX1

Sample Details

  1. Animals/Lines: Early Stage Embryos

External Links

  1. DCC-2539 Ahringer_L3_Worm_Samples_1
  2. DCC-2540 Ahringer_L3_Worm_Samples_2


Release Date: 2009-09-23