Post-Embryo Fax-sorted cells Signal
tiling arrays (Waterston project, Miller subgroup)
RNA was isolated from various post-embryonic stages and co-immunoprecipitation with reporter antibodies. This was amplified for application as a labeled, double stranded cDNA to the Affymetrix C. elegans 1.0 whole genome tiling array. A smoothed density plot (log 2 transformed with a window size of 110) of probe intensities and predicted transcriptionally active regions (TARs) are shown.
Series DescriptionOur experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, we are extracting RNA from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
Release Date: 2009-11-18