Adult spe-9 Day 8

spe-9 adult hermaphrodite 8 days post-L4 small ncRNA RNA-Seq (Waterston project, Slack subgroup)

Details

small RNAs (<200bp) were isolated from spe-9 adults 8 days post-L4, and sequenced using Illumina technology. Sequences were aligned to the WS180 genome using SOAP, and converted to SAM. Alignments were lifted to WS190 coordinates by the DCC.

Series Description

We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries for small RNAs. Each library was sequenced using recent advances in high-throughput sequencing technology, Solexa. This study should lead to a better understanding of the aging process and age-related diseases.

Protocols

  1. Growth and isolation: worm growth, worm staging and isolation, small RNA isolation, cDNA library prep and Illumina sequencing
  2. Data Analysis: SOAP alignment
  1. Growth Conditions: 23 degrees celsius

Sample Details

  1. Animals/Lines: Adult spe-9(hc88) 23dC 8 days post-L4 molt, BA671
  2. External Links: GSM463027, GSM463030


Release Date: 2010-02-13