fer-1 unfertilized oocytes
miRNA isolation and 454 sequencing from fer-1 unfertilized oocytes (Piano project, Kim subgroup)
small RNAs (<200 bp) were isolated from unfertilized oocytes derived from fer-1 fertilization-defective adults and sequenced on the Roche 454 platform, and aligned to the WS190 genome using MegaBlast.
The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.
Release Date: 2010-02-12