WormBase Tree Display for Expr_pattern: Expr2896
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| Expr2896 | Expression_of | Gene | WBGene00001080 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001080 | ||
| Expression_data | GO_term | GO:0000805 | |
| Subcellular_localization | Immunofluorescence experiments using DPY-21 antibodies showed DPY-21 to be diffusely localized in nuclei of XX embryos (<40 cells) that had not yet activated dosage compensation. In embryos with greater than 40 cells, DPY-21 formed punctate, subnuclear foci that coincided with the X-localized SDC-3 foci. The DPY-21 foci co-localized with X chromosomes by probing simultaneously with DPY-21 antibodies and X-chromosome-specific DNA probes that were visualized by fluorescence in situ hybridization (FISH). DPY-21 maintains its localization to X chromosomes throughout C. elegans development as indicated by its presence on X chromosomes of adult gut nuclei. The in vivo specificity of both DPY-21 antibodies was confirmed by the absence of staining in the two dpy-21 mutant strains homozygous for early nonsense mutations, dpy-21(e428) or dpy-21(y59). | ||
| XO male embryos was stained with DPY-21 antibodies and an X-chromosome FISH probe to confirm that DPY-21 is expressed in males and to determine whether it localizes to X, as it does in hermaphrodites. In XO embryos of all ages, the pattern of localization resembled that in XX embryos that had not yet activated dosage compensation. DPY-21 was dispersed throughout the nucleus, in multiple foci of intense staining that were not coincident with the X chromosome. | |||
| Type | Antibody | ||
| Picture | WBPicture0000007577 | ||
| Reference | WBPaper00006355 | ||
| Antibody_info | WBAntibody00000707 | ||
| WBAntibody00000708 |
