WormBase Tree Display for RNAi: WBRNAi00090277

WBRNAi00090277 Homol: Homol_homol: R12H7:RNAi
  Sequence_info: PCR_product: mv_AAB51534
  Experiment: Laboratory: CC
    Date: 15 Aug 2011 00:00:00
    Genotype: ccIs4251 [Pmyo-3::MitGFP, Pmyo-3::NLS::GFP-lacZ]
    Treatment: 3-4 L4 hermaphrodite worms were placed on an RNAi plate and incubated for 72-96 hours at 20 degrees Celsius. Young adult progeny were then scored for mitochondrial morphological defects/dystrophies. An additional examination of mitochondrial morphology was made in adults again 24 hours later. L4 hermaphrodites from the F1 generation were then transferred to newly seeded RNAi plates for examination of the F2 generation. Genes whose knockdown was observed to affect mitochondrial morphology in the F1 and F2 generations above were retested by exposing adult worms to an acute exposure of RNAi bacteria. In these experiments, worms were raised to young adulthood on non-RNAi bacteria at 20 degrees Celsius and then manually transferred to RNAi plates. Mitochondrial morphology was examined 24, 48, and 72 hours post transfer.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: R12H7.1a Inferred_automatically: RNAi_primary
      R12H7.1b Inferred_automatically: RNAi_primary
    Gene: WBGene00006749 Inferred_automatically: RNAi_primary
    Transcript: R12H7.1a.2 Inferred_automatically: RNAi_primary
      R12H7.1a.1 Inferred_automatically: RNAi_primary
      R12H7.1b.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00040284
  Phenotype: WBPhenotype:0001401 Remark: RNAi was observed to significantly perturb mitochondrial morphology in body wall muscles, as determined by observation of a muscle-specific fluorescent mitochondrial reporter. This affect was observed after chronic RNAi exposure (RNAi continuously for two generations), but not after acute exposure to RNAi only during adulthood.
      EQ_annotations: Anatomy_term: WBbt:0006804 PATO:0000460
  Remark: (Figure 2) unc-9 RNAi
  Method: RNAi