WormBase Tree Display for Antibody: WBAntibody00000353
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| WBAntibody00000353 | Summary | Rabbit polyclonal antiserum was raised against a region common to all isoforms and used to localize the protein in whole animals. | ||
|---|---|---|---|---|
| Public_name | [cgc4615]:cle-1 | |||
| Gene | WBGene00000527 | |||
| Isolation | Location | CH | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | Primers CE-3 (5 -ATAAGCTTATGGAGCAGCTGCTGATCCAGT-3 ) and CE-4 (5 -ATGGTACCTGATGATGGTGTTAGTATGGAA-3 ) were used to generate a 469-bp RT-PCR fragment that was cloned into KpnIHindIII-digested vector pQE-41 (QIAGEN), which adds an NH2-terminal His tag to amino acids 287 to 439 (CLE-1C). The resulting clone, cecolPQE-41, was transformed into Escherichia coli strain M15, and protein expression was performed according to manufacturer's instructions(QIAGEN). Purified fusion protein was used to immunize rabbits. The resulting antisera were affinity purified on columns carrying the purified fusion protein. | ||
| Animal | Rabbit | |||
| Expr_pattern | Expr2567 | |||
| Expr860 | ||||
| Reference | WBPaper00004615 | |||
| WBPaper00005871 |
