WormBase Tree Display for Construct: WBCnstr00004632
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| WBCnstr00004632 | Summary | [ceh-20::ceh-20-gfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00000443 | ||
| Gene | WBGene00000443 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | To generate a rescuing translational fusion CEH-20::GFP reporter gene, a 10 kb genomic fragment that contains 7 kb of the ceh-20 upstream regulatory sequence and almost the complete coding sequence was amplified with the following forward and reverse primers: 5'-ACA TGC ATG CAT GTA AGA GTG CGG ACG GTA GAG-3' and 5'-TCC CCC CGG GGG GAA GCA TTG TCC ATT TGT TGT TG-3'. The PCR product was cloned into the expression vector pPD95.75. swEx543 is able to rescue the early larval arrest phenotype caused by the putative null allele ay38. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00004650 | |
| Reference | WBPaper00028766 |
