WormBase Tree Display for Construct: WBCnstr00007890
expand all nodes | collapse all nodes | view schema
| WBCnstr00007890 | Summary | [Punc-119::pst-1a::egfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006843 | ||
| Gene | WBGene00004206 | ||
| Other_reporter | eGFP | ||
| Construction_summary | Plasmids were constructed on a pFX_EGFPT vector backbone. cDNAs were amplified by reverse transcription-PCR from total RNA of wild-type C. elegans with specific primers tagged with the NotI restriction site. We inserted the cDNA fragments digested with NotI into the Pdpy-7::egfp to create Pdpy-7::pst-1a::egfp. Pdpy-7::pst-1a::egfp was digested with NotI to yield the pst-1a which was cloned into Punc-119::egfp. | ||
| Used_for | Transgene_construct | WBTransgene00008170 | |
| Reference | WBPaper00036358 |
