WormBase Tree Display for Construct: WBCnstr00010561
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| WBCnstr00010561 | Other_name | Expr1987_Ex | |
|---|---|---|---|
| Summary | [eor-2::gfp] | ||
| Driven_by_gene | WBGene00001325 | ||
| Gene | WBGene00001325 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [eor-2::gfp] translational fusion. A rescuing eor-2::gfp reporter (pRH29) was made by first generating a rescuing clone (pRH27), which is a BamHIBglII fragment containing the entire eor-2 coding region with 3.8 kb of upstream sequence and 1.1 kb of downstream sequence cloned into pBSK(+). The GFP coding region from pPD102.33 (provided by A. Fire) was then cloned into a unique NruI site in pRH27, which results in the in-frame insertion of GFP(S65C) three residues before the stop codon. | ||
| Clone | pPD102.33 | ||
| Used_for | Transgene_construct | WBTransgene00027793 | |
| Reference | WBPaper00005344 |
