WormBase Tree Display for Construct: WBCnstr00010858
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| WBCnstr00010858 | Other_name | Expr2571_Ex | |
|---|---|---|---|
| Summary | [spl-1::gfp] | ||
| Driven_by_gene | WBGene00004981 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | [spl-1::gfp] promoter fusion. For transcriptional reporter constructs, a promoter region of 512 nucleotides of Y66H1B.4 upstream untranslated sequence corresponding to a position -3 to -515 relative to the ATG start codon was generated by PCR from T07A9 cosmid template DNA. Similarly, a promoter region of 3949 nucleotides of T07A9.1 upstream untranslated sequence corresponding to a position -2 to -3951 relative to the ATG start codon was generated by PCR from T07A9 cosmid template DNA using the primer pair PstIT07A9 and SmaIT07A9. Following restriction enzyme digestion, these PCR products were cloned into PstI and SmaI sites of the GFP reporter plasmids pPD95.75 and pPD95.67. | ||
| Clone | T07A9 | ||
| pPD95.75 | |||
| Used_for | Transgene_construct | WBTransgene00028086 | |
| Reference | WBPaper00005941 |
