WormBase Tree Display for Construct: WBCnstr00013269
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| WBCnstr00013269 | Other_name | Expr8345_Ex | |
|---|---|---|---|
| Summary | [swd-3::gfp] | ||
| Driven_by_gene | WBGene00006474 | ||
| Gene | WBGene00006474 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [swd-3::gfp] functional translational fusion with 270 bp of the promoter region of C14B1.4. For the swd-3::GFP construct, 270 bp of the promoter region of C14B1.4 was amplified from cosmid C14B1 using the primers wdrPf and wdrPr containing an XbaI and a BamHI site, respectively. The resulting fragment was inserted 5' of the GFP coding sequence of pPD117.01 vector by XbaIBamHI digestion. A 1600-bp fragment of the C14B1.4 including the entire coding region and its 3'UTR sequences was amplified using primers wdrGf and wdrGr containing an EcoR1and an ApaI site, respectively, and inserted into the equivalent sites in pPD117.01. The resulting construct was renamed pTS1. | ||
| Clone | pPD117.01 | ||
| C14B1 | |||
| Used_for | Transgene_construct | WBTransgene00030469 | |
| Reference | WBPaper00032309 |
