WormBase Tree Display for Construct: WBCnstr00013270
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| WBCnstr00013270 | Other_name | Expr8346_Ex | |
|---|---|---|---|
| Summary | [set-2::gfp] | ||
| Driven_by_gene | WBGene00004782 | ||
| Gene | WBGene00004782 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [set-2::gfp] translational fusion. For set-2::GFP, a 10400-bp NsiIEco47III fragment from cosmid C26E6 was subcloned into pGEM4 cut with PvuIIPstI to produce pTS3. A 395-bp promoter fragment upstream of the ATG start codon was amplified from pTS3 with primers Set2-pro1 and Set2-pro2 containing XbaI and XmaI sites and inserted in pPD117.01, upstream of GFP coding sequences to produce pSETa. A 255-bp fragment of the set-2 coding region, starting from the start ATG codon, was then amplified with primers Set2-pro3 and Set2-pro4 adding EcoRI and ApaI restriction sites and inserted downstream of the GFP coding sequences of pSETa to produce pSETb. pSETb was digested with SacII, and the 1300-bp fragment released, containing GFP flanked by set-2 promoter and coding regions, was inserted in pTS3 cut with the same enzyme, resulting in pTS4. | ||
| Clone | C26E6 | ||
| pPD117.01 | |||
| Used_for | Transgene_construct | WBTransgene00030470 | |
| Reference | WBPaper00032309 |
