WormBase Tree Display for Construct: WBCnstr00013709
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| WBCnstr00013709 | Other_name | Expr9084_Ex | |
|---|---|---|---|
| Summary | [ash-2p::ash-2::gfp] | ||
| Driven_by_gene | WBGene00012458 | ||
| Gene | WBGene00012458 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [ash-2p::ash-2::gfp] translational fusion. Pash-2::ash-2::gfp transgenic plasmid included 1.6 kb of ash-2 promoter driving the full-length ash-2 cDNA fused to GFP at the C terminus. The ash-2 promoter was amplified by PCR from wild-type (N2) genomic DNA using the following primers, F: 5'-ATAGCTAGCATCTCGTTCTTCTTCTGCTCACGG-3' and R: 5'-TATGTCGACAGTGCTGAAAATCTAGTTTTTTAG-3' and subcloned into pSM between NheI and AccI. The ash-2 cDNA isoform A (C. elegans ORFeome library, Open Biosystems) was amplified by PCR with the following primers, F: 5'-ATAGTCGACATGAGAAGCTCAAAAGGAGGTCGGGG-3' and R: 5'-TATGGTACCATAATTTCTTGTTTAATTTCTTTC-3' and cloned into pSM containing the ash-2 promoter, between AccI and KpnI. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00030894 | |
| Reference | WBPaper00036383 |
