WormBase Tree Display for Construct: WBCnstr00014290
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WBCnstr00014290 | Public_name | fUL#JW182 | |
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Other_name | Expr9770_Ex | ||
Summary | [C07A12.5::GFP; pRF4] | ||
Driven_by_gene | WBGene00005008 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | Clone = fUL#JW182. The reporter gene fusion assayed was made by recombineering WRM0610dC05. Sequence of homology arms on primers used to direct site of reporter insertion during recombineering, sequence for upstream of reporter first: catttgtataaatcatcaatcctatgttaatattatttcagtagaagacg, acctctccttgttcattcttcaactcctcaagtttgcgcttgcgaggtgg. gfp was inserted immediately after the spr-3 start codon. pRF4 cotransformant: pick rollers to maintain (not integrated). No other strains retained. --precise ends. | ||
Used_for | Transgene_construct | WBTransgene00031215 | |
Reference | WBPaper00040230 | ||
Person | WBPerson266 |