WormBase Tree Display for Construct: WBCnstr00019761
expand all nodes | collapse all nodes | view schema
WBCnstr00019761 | Public_name | pKS20 | |
---|---|---|---|
Summary | [nlp-3p::HID-1::GFP] | ||
Driven_by_gene | WBGene00003741 | ||
Gene | WBGene00001844 | ||
Fusion_reporter | GFP | ||
Construction_summary | The NM1699 construct, which contains the hid-1 promoter driving the genomic hid-1 coding region fused to eGFP, was a kind gift from the Nonet laboratory. The NM1699 construct was digested with KpnI and AatII to replace the native hid-1 promoter with various neuron-specific promoters: to drive sensory and pharyngeal specific expression, a 700-bp fragment upstream of the nlp-3 start codon was amplified and digested with KpnI-AatII to generate pKS20. | ||
Used_for | Transgene_construct | WBTransgene00020284 | |
Reference | WBPaper00045598 |