WormBase Tree Display for Construct: WBCnstr00019842
expand all nodes | collapse all nodes | view schema
| WBCnstr00019842 | Other_name | Expr1887_Ex | |
|---|---|---|---|
| Summary | [ptp-3B::gfp] | ||
| Driven_by_gene | WBGene00004215 | ||
| Gene | WBGene00004215 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [ptp-3B::gfp] translational fusion. To create the ptp-3B::GFP minigene, a unique Pst I site in the ptp-3B cDNA immediately 3' to the coding region for the second phosphatase domain was used to insert GFP coding sequence (GFP variant F64L S65T) in frame with the PTP-3B protein (clone pBH8). A 14651 bp NcoI-NotI fragment from cosmid F38A3, corresponding to the ptp-3B promoter and exons 14-20 was cloned into pSL1190 (pBH1). From pBH8 a 5 kb NotI fragment was cloned into the NotI site of pBH1. The resulting clone, pCZ406, contains genomic sequence for the promoter and the first approx. 6.5 exons of PTP-3B; the second half of exon 19 and subsequent exons are present as cDNA, tagged with GFP. --precise ends. | ||
| Used_for | Transgene_construct | WBTransgene00031651 | |
| Reference | WBPaper00005259 |
