WormBase Tree Display for Construct: WBCnstr00021201
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WBCnstr00021201 | Summary | [mec-3p::unc-62b] | |
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Driven_by_gene | WBGene00003167 | ||
Gene | WBGene00006796 | ||
Construction_summary | A 2.4 kb mec-3 promoter was cloned from wild type (N2) genomic DNA into the Gateway pDONR221 P4-P1r vector. The cDNA of unc-62b was cloned into Gateway pDONR221. The resulted entry vectors, together with the appropriate 5' entry vector, the pENTR-unc-54-3' UTR and destination vector pDEST-R4-R3 were used in the LR reaction to create the final expression vectors. The Gateway cloning method can be found at http: | ||
Used_for | Transgene_construct | WBTransgene00021536 | |
WBTransgene00021537 | |||
Reference | WBPaper00048760 |