WormBase Tree Display for Construct: WBCnstr00022934
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WBCnstr00022934 | Summary | [unc-119::egl-30] | |
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Driven_by_gene | WBGene00006843 | ||
Gene | WBGene00001196 | ||
Construction_summary | Full-length egl-30 genomic DNA was amplified by PCR from wild-type worms using the upstream primer 5'-ATGGCCTGCTGTTTATCCGAAGAG-3' and the downstream primer 5'-TTACACCAAGTTGTACTCCTTCAGATTATGCTGTAGAAT-3'. The PCR product was blunt-end ligated into the BamHI site of the unc-119 promoter plasmid pBY103 (a gift from M. Maduro), making pR30 (unc-119::egl-30). | ||
Used_for | Transgene_construct | WBTransgene00031981 | |
Reference | WBPaper00006284 |