WormBase Tree Display for Construct: WBCnstr00042805
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WBCnstr00042805 | Public_name | pBC1696 |
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Summary | Pmai-2::dCas9::SV40-NLS::HA-TAG::GS-Linker::SV40-NLS::GFP::GFP::SV40-NLS::mai-2 3'UTR in pCFJ350 | |
Driven_by_gene | WBGene00015248 | |
UTR_3 | WBGene00015248 | |
Fusion_reporter | GFP | |
Other_reporter | dCas-9 | |
Purification_tag | HA-tag | |
Construction_summary | we fused the Streptococcus pyogenes dCas9 coding region to two copies of the EGFP coding region, flanked by nuclear localization sequences (NLS). The resulting gene fusion was put under the control of a heat-inducible promoter (Phsp-16.41dCas9::NLS::2xEGFP::NLS). | |
Reference | WBPaper00064743 |