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WormBase Tree Display for Construct: WBCnstr00042805

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Name Class

WBCnstr00042805Public_namepBC1696
SummaryPmai-2::dCas9::SV40-NLS::HA-TAG::GS-Linker::SV40-NLS::GFP::GFP::SV40-NLS::mai-2 3'UTR in pCFJ350
Driven_by_geneWBGene00015248
UTR_3WBGene00015248
Fusion_reporterGFP
Other_reporterdCas-9
Purification_tagHA-tag
Construction_summarywe fused the Streptococcus pyogenes dCas9 coding region to two copies of the EGFP coding region, flanked by nuclear localization sequences (NLS). The resulting gene fusion was put under the control of a heat-inducible promoter (Phsp-16.41dCas9::NLS::2xEGFP::NLS).
ReferenceWBPaper00064743