WormBase Tree Display for Expr_pattern: Expr8160
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Expr8160 | Expression_of | Gene | WBGene00001443 | ||
---|---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00001443 | ||||
Expression_data | Life_stage | WBls:0000024 | |||
WBls:0000038 | |||||
WBls:0000027 | |||||
WBls:0000035 | |||||
WBls:0000041 | |||||
WBls:0000015 | |||||
WBls:0000004 | |||||
WBls:0000021 | |||||
WBls:0000010 | |||||
WBls:0000013 | |||||
Anatomy_term | WBbt:0004292 | Uncertain | |||
Remark | rectum muscle | ||||
WBbt:0004506 | Certain | ||||
WBbt:0004520 | Certain | ||||
WBbt:0005319 | Certain | ||||
WBbt:0005451 | Certain | ||||
WBbt:0005798 | Uncertain | ||||
Remark | rectum muscle | ||||
WBbt:0005821 | Certain | ||||
WBbt:0005824 | Uncertain | ||||
Remark | proctodeum muscle | ||||
WBbt:0005825 | Uncertain | ||||
Remark | proctodeum muscle | ||||
WBbt:0005826 | Uncertain | ||||
Remark | proctodeum muscle | ||||
WBbt:0005827 | Uncertain | ||||
Remark | proctodeum muscle | ||||
GO_term | GO:0030424 | ||||
GO:0005737 | |||||
GO:0055120 | |||||
GO:0005884 | |||||
Subcellular_localization | The GFP was localized mainly in the cytoplasm. The fli-1 expression in the spermatheca was unevenly distributed with strong expression in the distal and proximal part of spermatheca. fli-1 was also expressed within neuronal migratory structures such as axons. In the body-wall muscle, fli-1::gfp was localized in a distinct striated pattern, with accumulation of FLI-1::GFP at dense bodies, which is similar to the Z-disc in the muscle cells in other organisms. The expression pattern of fli-1 in body-wall muscle cells is reminiscent of the distribution of the actin filaments. Authors stained F-actin in body-wall muscle cells with rhodamine-labeled phalloidin and found that it colocalized with FLI-1::GFP. | ||||
Type | Reporter_gene | ||||
Pattern | The reporter was widely expressed and the expression level was dynamic, varying among tissues and developmental stages. The expression of fli-1::gfp was first detected during embryogenesis before the apparent morphogenesis occurred. In the comma-stage embryo, expression of fli-1::gfp was detected in muscle cells. The expression of fli-1::gfp was not observed before the 32-cell-stage embryos (n > 40 embryos examined). At post-embryonic stages, expression of fli-1::gfp was detected in many tissues, including the pharyngeal muscle, rectum muscle, vulva muscle, proctodeum muscle in males, and somatic gonad tissues (including spermatheca and distal tip cell). | ||||
Picture | WBPicture0000010969 | ||||
Remark | Picture: Figure 9. | ||||
Reference | WBPaper00030927 | ||||
Transgene | WBTransgene00030350 |