WormBase Tree Display for Expr_pattern: Expr10026

Expr10026 Expression_of: Gene: WBGene00000549
    Reflects_endogenous_expression_of: WBGene00000549
  Expression_data: Life_stage: WBls:0000003
    Anatomy_term: WBbt:0007028 Certain
    GO_term: GO:0072686
      GO:0005634
      GO:0005737
      GO:0005813
      GO:0000776
  Type: Antibody
  Pattern: CLS-2 first appeared on the centrosomes at prophase in some embryos. By prometaphase, centrosome staining was evident in most embryos and the signal also entered the nucleus. CLS-2 localized to the kinetochore interfaces and on the spindle at metaphase in one-cell embryos, in addition to the centrosomes. Spindle staining persisted into anaphase, especially in the mid spindle region; anaphase spindle staining was not reported in C. elegans mitotic embryos previously, but was shown for meiotic spindles. Centrosome staining also persisted through anaphase and into telophase. All embryos also showed cytoplasmic staining which was absent after RNAi depletion. In multicellular embryos, interphase cells showed strong cytoplasmic staining that appeared enriched at cell contacts but did not appear to colocalize with regions of the cells showing the densest tubulin staining. Mitotic cells in multicellular embryos exhibited centrosome, kinetochore and spindle staining through at least the 28-cell stage.
  Reference: WBPaper00041121
  Antibody_info: WBAntibody00002342