WormBase Tree Display for Expr_pattern: Expr10041
expand all nodes | collapse all nodes | view schema
| Expr10041 | Expression_of | Gene | WBGene00007732 | Inferred_automatically |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00007732 | |||
| Expression_data | Life_stage | WBls:0000038 | ||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| Anatomy_term | WBbt:0005784 | Certain | ||
| WBbt:0006798 | Certain | |||
| Type | In_situ | Digoxigenin-labeled, single-stranded sense and antisense probes were generated from a 1.3 kb spe-44 cDNA fragment by linear amplification according to the manufacturer's protocol (Roche). | ||
| Pattern | In situ hybridizations of dissected gonads were performed to ascertain the precise spatial pattern of spe-44 expression. The fem- 3(gf) and fem-1(lf) mutations were used to restrict hermaphrodite gametogenesis to sperm or oocyte production, respectively. Abundant expression was first detected in fem-3(gf) animals during the L3 stage in the early meiotic germline. DAPI staining of the chromatin indicated that expression coincides with early pachytene of meiosis I. By contrast, spe-44 expression was undetectable in fem-1(lf) germlines at any stage of development. Thus, spe-44 expression is restricted to the sperm-producing germline at a time and place consistent with the regulation of sperm gene expression. | |||
| Picture | WBPicture0000012718 | |||
| Reference | WBPaper00041071 | |||
| Historical_gene | WBGene00045485 | Note: This object originally referred to WBGene00045485. WBGene00045485 is now considered dead and has been merged into WBGene00007732. WBGene00007732 has replaced WBGene00045485 accordingly. |
