WormBase Tree Display for Expr_pattern: Expr10610

Expr10610 Expression_of: Gene: WBGene00007918
    Reflects_endogenous_expression_of: WBGene00007918
  Expression_data: Anatomy_term: WBbt:0005274 Certain
      WBbt:0005278 Certain
      WBbt:0005733 Certain
      WBbt:0005772 Certain
      WBbt:0005775 Certain
      WBbt:0005813 Certain
      WBbt:0006751 Partial
        Remark: Expressed in some neurons in the head and tail.
      WBbt:0006759 Partial
        Remark: Expressed in some neurons in the head and tail.
    GO_term: GO:0005739
      GO:0030425
      GO:0042734
      GO:0005794
      GO:0043025
      GO:0048786
      GO:0016020
  Type: Reporter_gene
  Pattern: Transgenic animals expressing green fluorescent protein (GFP) under the control of a fully rescuing sphk-1 promoter fragment displayed fluorescence in multiple tissues, including the hypodermis (skin), muscles, the intestine, excretory canal cells, and some neurons in the head and tail.In DA/DB class cholinergic motor neurons, functional GFP-tagged SPHK-1 fusion proteins (SPHK-1-GFP) were detected in extranuclear regions in cell bodies, likely corresponding to Golgi (Sumakovic et al., 2009), occasionally in puncta in dendrites (data not shown), and in puncta at en passant presynaptic terminals (Chan et al., 2012). Colocalization studies revealed that SPHK-1-GFP was closely apposed to but not colocalized with the synaptic vesicle (SV) and actin-associated protein SNN-1/synapsin. In body wall muscles, SPHK-1-GFP was primarily observed on the outer surface of mitochondria as demonstrated by its colocalization with an RFP-tagged outer membrane mitochondrial marker. Thus, SPHK-1 appears to localize to different cellular membranes depending on cell type.SPHK-1 associates with presynaptic membranes at release sites, but not synaptic vesicles.
  Reference: WBPaper00041824
  Transgene: WBTransgene00018005