WormBase Tree Display for Expr_pattern: Expr10737
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| Expr10737 | Expression_of | Gene | WBGene00003001 |
|---|---|---|---|
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000027 | |||
| WBls:0000035 | |||
| WBls:0000018 | |||
| WBls:0000019 | |||
| WBls:0000020 | |||
| WBls:0000017 | |||
| WBls:0000021 | |||
| Anatomy_term (18) | |||
| Type | Cis_regulatory_element | ||
| Reporter_gene | |||
| Pattern | lin-12(1in_1408) displayed a highly differentiated, characteristic expression pattern in the VPC lineages. The expression was already evident in comma-stage embryos. Several glowing cells of different types were seen along the anteroposterior body axis at the twofold embryonic stage. At the L1 stage, lin-12(1in_1408) was expressed in all Pn.p cells. Some unidentified cells in the head and tail regions were also gfp-positive at this stage. By the early L2 stage, lin-12(1in_1408) expression remained strong only in the P(3-8).p cells. Unlike the other Pn.p cells, they remain unfused with the hypodermal syncytium, thereby retaining their competence to differentiate into a vulval cell type. After vulval induction at the early L3 stage, lin-12(1in_1408) was strongly expressed in P5.p and P7.p, the two VPCs that adopt 2nd vulval fates, but weakly detectable in the other VPCs, P(3-4,6,8).p. As shown earlier (Wilkinson and Greenwald, 1995; 276 Levitan and Greenwald, 1998), lin-12 expression remained on in the P5.p and P7.p granddaughters by the late L3 stage. In addition to the P5.p and P7.p descendants, we found obvious fluorescence in the daughters of other VPCs, and even in the P6.p granddaughters [the daughters of P(3,4,8).p fuse with the hypodermis]. Nevertheless, expression of lin-12(1in_1408) was constantly intense in the P5.p and P7.p lineages, as compared with other VPC lineages. At the L2 and early L3 stages, lin-12(1in_1408) is also expressed in the two AC/VU precursors. Later, when lin-12(1in_1408) expression became increased in P5.p and P7.p relative to the other VPCs, transgene activity was seen in the presumptive VU cell, but not in the AC. During the L4 stage, expression of lin-12(1in_1408) was gradually lost from the P6.p lineage; only the P(5,7).p descendants retained GFP fluorescence in the developing vulval tissue. It is worth to note that lin-12(1in_1408) was also active in certain hypodermal and intestinal cells throughout larval development, presumably due to background expression of the vector. In adult hermaphrodites, lin-12(1in_1408) expression could not be detected. The only exception was presented by a few unidentified cells in the posterior body part, probably due to background expression. | ||
| Picture | WBPicture0000013069 | ||
| Reference | WBPaper00041920 | ||
| Transgene | WBTransgene00018123 |
