WormBase Tree Display for Expr_pattern: Expr1078

Expr1078 Expression_of: Gene: WBGene00004027
    Reflects_endogenous_expression_of: WBGene00004027
  Homol: Homol_homol: Y49E10:Expr
  Expression_data: Life_stage: WBls:0000005
    Anatomy_term: WBbt:0004422 Certain
      WBbt:0006873 Certain
      WBbt:0006874 Certain
      WBbt:0006875 Certain
    GO_term: GO:0005634
      GO:0005737
      GO:0005813
      GO:0005829
      GO:0043186
  Subcellular_localization: In embryos, PIE-1:GFP was found predominantly in the cytoplasm and nuclei of germline blastomeres. In the cytoplasm, PIE-1:GFP was present both diffusely throughout the cytosol and at higher concentration on P granules. PIE-1:GFP also appeared to associate with discrete foci in nuclei. The identity of these foci is not known. In oocytes and newly fertilized embryos, PIE-1:GFP was present uniformly throughout the cytoplasm. In the late 1-cell stage after the pronuclei have formed, PIE-1:GFP levels began to decrease in the anterior and increase in the posterior. By pronuclear meeting, PIE-1:GFP was found predominantly in the posterior. During mitosis, PIE-1:GFP also accumulated on both centrosomes with higher levels on the posterior centrosome. As a result of this asymmetric enrichment, most of the PIE-1:GFP was inherited by the posterior blastomere P1 during the first cleavage. In P1, P2, and P3, PIE-1:GFP distribution followed a sequence similar to that observed in the zygote, with the exception that PIE-1:GFP became increasingly more nuclear during each interphase. Before each cell division, PIE-1:GFP in the cytoplasm decreased on the side of the cell destined for the next somatic blastomere. At the start of mitosis, PIE-1:GFP disappeared from the nucleoplasm and became associated with centrosomes at both ends of the newly formed spindle. As mitosis progressed, PIE-1:GFP levels in the cytoplasm continued to decrease on the somatic side of the cell; concomitantly PIE-1:GFP levels decreased on the centrosome destined for the somatic daughter and increased on the centrosome destined for the germline daughter. After cytokinesis, most PIE-1:GFP was found in the germline daughter with only low levels left in the somatic daughter (e.g., EMS in the 4-cell stage). PIE-1:GFP fluorescence diminished progressively in that cell and was not detected in its progeny.
  Type: Reporter_gene
  Pattern: Expressed in blastula embryos, not detailed description on the expression in later stages.
  Picture: WBPicture0000010074
  Remark: This fusion is functional, as it can rescue the embryonic lethality of a pie-1(0) mutant. In all 14 lines examined, GFP fluorescence in the adult germline and in embryos was observed in a pattern identical to that reported for PIE-1 using immunolocalization.
  Reference: WBPaper00004304
  Transgene: WBTransgene00027331