WormBase Tree Display for Expr_pattern: Expr1097
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| Expr1097 | Expression_of | Gene | WBGene00004831 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004831 | ||||
| Expression_data | Anatomy_term | WBbt:0005409 | Partial | ||
| Remark | Expressed in some motor neurons. | ||||
| WBbt:0005451 | Uncertain | ||||
| Remark | possible | ||||
| WBbt:0005813 | Certain | ||||
| WBbt:0005821 | Certain | ||||
| WBbt:0006749 | Certain | ||||
| Type | Reporter_gene | ||||
| Pattern | Transformed progeny expressed the slo-2:GFP fusion protein in many tissues including body-wall and vulva muscle (and possibly in pharyngeal muscle), processes of the nerve ring, and in some motor neurons. | ||||
| Remark | This construct, lacking the slo-2 initiator methionine and upstream promoter, was co-injected together with the F08B12 cosmid containing the entire slo-2 gene and its promoter into the syncitial gonad of adult hermaphrodite N2 nematodes. Recombination between the slo-2:GFP construct and the cosmid was necessary for slo-2:GFP gene expression, and was enhanced by linearization of the slo-2:GFP segment and the cosmid at appropriate sites31. Control injections with the slo-2:GFP segment alone produced no GFP signal. | ||||
| Reference | WBPaper00004344 | ||||
| Transgene | WBTransgene00027343 |
