WormBase Tree Display for Expr_pattern: Expr11655
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Expr11655 | Expression_of (2) | |
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Expression_data (2) | ||
Subcellular_localization | GFP::UNC-60A localized in punctate structures at the AC-basement membrane interface before invasion. To determine whether these structures were invadopodia, their localization was examined relative to F-actin and basement membrane breaching. A tight correlation between F-actin localization and UNC-60A enrichment at the AC-basement membrane interface was observed, as well as localization to the invadopodium that breached the basement membrane. Further, UNC-60A localization to the AC-basement membrane interface was dependent on the integrin INA-1/PAT-3, whose activity is required for invasion and promotes the localization of other invadopodia constituents. Thus, UNC-60A is a component of AC invadopodia. | |
Type | Reporter_gene | |
Pattern | At the time of invasion GFP::UNC-60A was present throughout the uterine tissue and at elevated levels in the anchor cell (1.35-fold greater levels versus neighboring uterine cells; n = 11 animals examined). GFP::UNC-60A was also more enriched at the invasive cell membrane (1.29-fold enrichment vs. 0.75-fold for neighboring uterine cells; n = 11 animals examined). | |
Picture | WBPicture0000013663 | |
Reference | WBPaper00045029 | |
Transgene (3) |