WormBase Tree Display for Expr_pattern: Expr11851
expand all nodes | collapse all nodes | view schema
| Expr11851 | Expression_of | Gene | WBGene00006888 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006888 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| WBbt:0008378 | Certain | |||
| GO_term | GO:0005737 | |||
| GO:0043186 | ||||
| GO:0048471 | ||||
| Subcellular_localization | Extruded gonads and embryos from xmSi05 adults (soma and germline expression) were placed on a slide and observed under an epifluorescence microscope to analyze the location of the transgene. The authors have previously reported that VBH-1 is expressed in cytoplasmic foci, perinuclear germ granules termed P granules in germ cells, and diffusely expressed throughout the cytoplasm of all blastomeres [WBPaper00031012]. The GFP transgene showed the same expression as the endogenous VBH-1 in germline cells and embryos suggesting that the expression of the transgene was similar to endogenous VBH-1. | |||
| Type | Reporter_gene | |||
| Pattern | the Pvbh-1::GFP::vbh-1::vbh-1 reporter showed high expression in both soma and germline. GFP::VBH-1 expression from line xmSi06 was observed in the cytoplasm of intestinal cells, with a clear enrichment in the apical pole in control animals. Although xmSi06 animals show somatic vbh-1 expression the authors cannot discard that this is an artifact of this transgene. | |||
| Picture | WBPicture0000013716 | |||
| Reference | WBPaper00045379 | |||
| Transgene | WBTransgene00020072 | |||
| WBTransgene00020073 |
