WormBase Tree Display for Expr_pattern: Expr1192
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| Expr1192 | Expression_of | Gene | WBGene00002915 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002915 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000013 | ||||
| Anatomy_term | WBbt:0003681 | Certain | ||
| WBbt:0005733 | Certain | |||
| Type | In_situ | Probes were visualized using an alkaline-phosphatase-conjugated anti-digoxigenin antibody.In situ hybridization was done using a myosin heavy chain probe to mark the position of the muscle cells and to show that the muscle cells are accessible to DNA probes under the conditions used. | ||
| Pattern | First detected in embryos before elongation. Signal is detected in two groups of cells: one group on the dorsal surface of the embryo flanking the dorsal midline, in the position of the dorsal hypodermal cells; and second group on the lateral edges of the embryo about midway between the dorsal and ventral surfaces, in the position of the ventral hypodermal cells. Later in development, at about the 1.51.75-fold stage, myotactin message is also detected in the pharynx. Myotactin message could not be detected in muscle cells. When myosin message is first detected, the positive cells are positioned midway between the dorsal and ventral surfaces, away from the lateral edges of the embryo. Later in development, the myotactin probe is detected in hypodermal cells at the dorsal and ventral edges of the embryo. At this stage, myosin is detected in cells positioned more interiorly. No evidence that myotactin is expressed in body wall muscle cells at any developmental stage. | |||
| Picture | WBPicture0000008922 | |||
| Reference | WBPaper00003643 |
