WormBase Tree Display for Expr_pattern: Expr12001
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| Expr12001 | Expression_of | Gene | WBGene00003026 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003026 | ||||
| Expression_data | Life_stage | WBls:0000041 | Anatomy_term | WBbt:0006796 | |
| WBbt:0006797 | |||||
| WBbt:0007849 | |||||
| WBls:0000669 | |||||
| Anatomy_term | WBbt:0006796 | Certain | |||
| Life_stage | WBls:0000041 | ||||
| Remark | Expression restricted to germ cells committed to oogenesis. | ||||
| WBbt:0006797 | Certain | ||||
| Life_stage | WBls:0000041 | ||||
| Remark | Expression restricted to germ cells committed to oogenesis. | ||||
| WBbt:0007849 | Certain | ||||
| Life_stage | WBls:0000041 | ||||
| Remark | Expression restricted to germ cells committed to oogenesis. | ||||
| GO_term | GO:0005737 | ||||
| Subcellular_localization | LIN-41 is strongly expressed in adult hermaphrodite germ cells beginning in mid-pachytene. LIN-41 protein levels rise dramatically at this time, increasing several-fold over a distance corresponding to just a few cell diameters. The timing of this rise consistently occurs just before the peak of MPK-1 mitogen-activated protein kinase (MAPK) activation in mid-pachytene, which may promote the progression of oogenic germ cells from early to late pachytene (Lee et al. 2007). LIN-41 is also abundantly expressed in diplotene and diakinesis-stage oocytes, but is generally reduced in abundance in the -1 oocyte as it undergoes meiotic maturation (14/17 oocytes identified), suggesting that LIN-41 may be eliminated at this time. Consistent with this interpretation, an N-terminal GFP::LIN-41 fusion protein, generated by CRISPR-Cas9 genome editing of the endogenous locus, is expressed in oocytes, disappears during meiotic maturation, and is absent from early embryos. Most LIN-41 is diffusely cytoplasmic, with a small amount of punctate LIN-41 that is most evident during pachytene. LIN-41 was also not detected in the germ lines of males or mid-L4 stage larvae, both of which have late pachytene-stage germ cells undergoing spermatogenesis, suggesting that pachytene-stage LIN-41 expression is restricted to germ cells committed to oogenesis. The primary, and possibly exclusive, site of LIN-41 expression in adults appears to be the oogenic germ line. LIN-41 expression in the C. elegans germ line is strongly associated, both developmentally and spatially, with the oogenic fate. | ||||
| Type | Antibody | ||||
| Picture | WBPicture0000013793 | ||||
| Reference | WBPaper00045803 | ||||
| Antibody_info | WBAntibody00002566 | ||||
| WBAntibody00002567 |
