WormBase Tree Display for Expr_pattern: Expr13274
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| Expr13274 | Expression_of | Gene | WBGene00008968 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00008968 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0005737 | |||
| GO:0000932 | ||||
| Subcellular_localization | To observe the sub-cellular localization of ELLI-1 protein, CRISPR was used to place a C-terminal GFP-3xFLAG tag on endogenous elli-1. A diffuse GFP signal can be observed at low levels in the germline of these living worms, and M2 FLAG antibody shows diffuse ELLI-1 expression in germline cytoplasm with some small ELLI-1 foci. While 4.3% of these foci can be found docked next to P granules, the majority of ELLI-1 foci accumulate in the rachis (averaged from 10 germlines). Within the rachis, ELLI- 1 foci partially overlap with the P-body component CGH-1, suggesting these foci are associated with RNA. We sought to determine whether ELLI-1 colocalizes with PATR-1, a P-granule component found only in P bodes but not P granules, but an available PATR-1 antibody did not work in dissected germlines, and a CRISPR-derived PATR-1::GFP strain generated for this study was surprisingly diffuse in the adult germline and did not form P-body-like foci. Zygotic ELLI-1 begins to accumulate in the cytoplasm of primordial germ cells between the comma to 2-fold stage of embryogenesis, but again appears distributed throughout the germ plasm instead of concentrated on P granules. | |||
| Type | Genome_editing | |||
| Reporter_gene | ||||
| Picture | WBPicture0000014509 | |||
| Reference | WBPaper00050743 |
