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WormBase Tree Display for Expr_pattern: Expr1395

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Name Class

Expr1395Expression_ofGeneWBGene00000238
Reflects_endogenous_expression_ofWBGene00000238
Expression_dataLife_stageWBls:0000024
WBls:0000027
WBls:0000035
Anatomy_termWBbt:0004409Certain
WBbt:0005175Certain
WBbt:0005753Certain
WBbt:0008112Certain
WBbt:0008117Certain
WBbt:0008121Certain
WBbt:0008125Certain
WBbt:0008129Certain
WBbt:0008133Certain
GO_termGO:0005634
GO:0005737
GO:0005913
Subcellular_localizationIn the vulval precursor cells, BAR-1 is predominantly cytoplasmic, with some protein present in the nucleus and some at cell junctions.
TypeAntibodyAffinity-purified rabbit polyclonal antibodies to a recombinant BAR-1 fusion protein produced in E. coli.
Reporter_gene
PatternSimilar expression patterns were obtained using either the BAR-1 antibodies in immunocytochemistry experiments or by direct observation of the functional BAR-1-GFP. BAR-1 is present in P3.p through P8.p in the late L1/early L2 stage and disappears in the mid-L3 stage before the first divisions of P3.p-P8.p. Did not observe BAR-1 in P1.p, P2.p, P9.p, P10.p or P11.p at this time. P6.p occasionally showed higher BAR-1 expression than the other vulval precursor cells, and was often observed to be the last vulval precursor cell to lose BAR-1 staining. In addition to the vulval precursor cells, BAR-1 is expressed in several other postembryonic cells, including the seam cells , cells in the somatic gonad and P12.
PictureWBPicture0000007989
ReferenceWBPaper00003200
TransgeneWBTransgene00027502
Antibody_infoWBAntibody00000191