WormBase Tree Display for Expr_pattern: Expr1444
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| Expr1444 | Expression_of | Gene | WBGene00004077 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004077 | ||
| Expression_data | Life_stage | WBls:0000024 | |
| WBls:0000003 | |||
| WBls:0000038 | |||
| WBls:0000027 | |||
| WBls:0000035 | |||
| Anatomy_term (12) | |||
| GO_term | GO:0005634 | ||
| Subcellular_localization | nucleus | ||
| Type | Antibody | Monoclonal mouse antibody against recombinant POP-1 protein. Immunolocalization results described in this paper are with mABRL2. | |
| Pattern | Between the 2-cell and 28-cell stages of embryogenesis, equal levels of POP-1 staining were detected in sister cells born from transverse cleavages. However, different levels of POP-1 stained in almost all pairs of sister cells resulting from an a/p cleavage. For example, in the AB lineage there are no POP-1 differences after the first two divisions, which are transverse, but there are differences after the third division, which is a/p. After the first a/p division in the AB lineage, as well as after a/p divisions in other lineages, authors invariably see higher levels of staining in the nucleus of the anterior sister than in the nucleus of its posterior sister. POP-1 asymmetry appears in interphase nuclei, then POP-1 staining diminishes and is not observed in prophase nuclei. The only anterior cell in which authors fail to detect POP-1 is the P4 blastomere. Heterogeneity in POP-1 staining is observed between many neighboring nuclei in embryos after the 28-cell stage. The descendants of the E lineage can be identified readily in fixed embryos at all stages; POP-1 asymmetry was found after the first, third, and fourth divisions of the E lineage, which are a/p. The second division of the E lineage is transverse (left/right), and symmetrical levels of POP-1 were found in both pairs of sister blastomeres. In late-stage embryos, POP-1 is prominent in the developing nervous system but absent from some other tissues like the hypodermis. In larvae during postembryonic development, POP-1 is present in the row of hypodermal cells, called seam cells, along the lateral surfaces of the body. POP-1 asymmetry is observed after the seam cells divide a/p; in each pair of sisters, POP-1 appears at higher levels in the anterior sister than in the posterior sister. In addition, POP-1 is detected in migratory cells called the Q neuroblasts and in the developing gonad and vulva. | ||
| Picture | WBPicture0000009512 | ||
| WBPicture0000009513 | |||
| Remark | No detailed description on cellular expression pattern at later stages. | ||
| Reference | WBPaper00002998 | ||
| Antibody_info | WBAntibody00000162 |
