WormBase Tree Display for Expr_pattern: Expr1446
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| Expr1446 | Expression_of | Gene | WBGene00003850 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00003850 | |||
| Expression_data | Anatomy_term | WBbt:0003826 | Certain | |
| WBbt:0003827 | Certain | |||
| WBbt:0003828 | Certain | |||
| WBbt:0003829 | Certain | |||
| WBbt:0003830 | Certain | |||
| WBbt:0003831 | Certain | |||
| WBbt:0003889 | Certain | |||
| WBbt:0003890 | Certain | |||
| WBbt:0003999 | Certain | |||
| WBbt:0004003 | Certain | |||
| GO_term | GO:0043025 | |||
| GO:0097730 | ||||
| Subcellular_localization | The ODR-3 protein was detected in cell bodies as well as cilia. | |||
| Type | Antibody | Rabbit polyclonal antibodies against the ODR-3 protein were generated and used to detect the endogenous ODR-3 protein in wild-type and mutant animals. | ||
| Pattern | The antiODR-3 antibodies stained only the ciliated endings of a few amphid neurons in wild-type animals. The AWC neurons were identified by the distinctive morphology of their ciliated endings, but the identity of the other cells expressing odr-3 could not be established based on cilia morphology. However, the overall pattern of staining is consistent with the predicted pattern based on the GFP fusion gene, and no staining was observed outside the amphid region. To help identify the cells that expressed ODR-3, the antiODR-3 antibodies were also used to stain animals with high copy overexpression of the odr-3 genomic clone. In these animals, the ODR-3 protein was detected in cell bodies as well as cilia, in a cluster of cells that appeared to be the AWA, AWB, AWC, ADF, and ASH cells that expressed odr-3::GFP transgenes. | |||
| Picture | WBPicture0000009586 | |||
| Reference | WBPaper00002996 | |||
| Antibody_info | WBAntibody00000161 |
