WormBase Tree Display for Expr_pattern: Expr15014
expand all nodes | collapse all nodes | view schema
| Expr15014 | Expression_of | Gene | WBGene00021832 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00021832 | |||
| Expression_data | Anatomy_term | WBbt:0005784 | Certain | |
| GO_term | GO:0000795 | |||
| Subcellular_localization | The expression of SYP-5 and SYP-6 was germline specific, and they exhibited distinct expression patterns. SYP-5::GFP reached its highest expression level at the end of the pachytene stage, while SYP-6::GFP exhibited strong expression before late pachytene. Moreover, the expression pattern of endogenous SYP-5 was similar to that observed for SYP-5::GFP, as evaluated with a SYP-5-specific antibody. Super-resolution structured illumination microscopy (SIM) analysis showed that both SYP-5::GFP and SYP-6::GFP located at the central region of the SC, where they were clearly flanked by the axis- associated HTP-3. While SYP-6::GFP was detected only during early prophase (until late pachytene), SYP-5::GFP colocalized with the SC throughout the meiotic prophase, and its signal disappeared by the end of diakinesis. By normalizing fluorescence signal intensities for different SYPs according to their levels at the mid-pachytene stage, we found that SYP-5::GFP loading was significantly lower than the other SYPs during early prophase (transition zone [TZ] and early pachytene), whereas SYP-6::GFP loading was lower than the other SYPs during late pachytene. These observations indicate that the composition of the SC varies during meiotic progression. | |||
| Type | Antibody | |||
| Reference | WBPaper00059491 | |||
| Antibody_info | WBAntibody00002890 |
