WormBase Tree Display for Expr_pattern: Expr15903
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| Expr15903 | Expression_of | Gene | WBGene00004051 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004051 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0005694 | |||
| GO:0005654 | |||
| Subcellular_localization | Immunofluorescence analyses showed that PARG-1::GFP is first detected in premeiotic and leptotene/zygotene nuclei and then became progressively enriched along chromosomes throughout pachytene. In late pachytene, PARG-1::GFP showed retraction toward the short arm of the bivalent (a chromosomal subdomain formed in response to CO formation) which was particularly evident at diplotene. In nuclei at the diakinesis stage, PARG-1::GFP was detectable mostly in the nucleoplasm. Co-staining with axial proteins HTP-1/ HTP-3 and the central SC component SYP-138,39,48 revealed recruitment of PARG-1::GFP onto synapsed chromosomes and confirmed its retraction to the short arm of the bivalent in late pachytene cells, which also harbors the chiasma and the central elements of the SC49,50. Overlapping localization of PARG-1::GFP with both the CO-promoting factor COSA-1 and SYP-1 further proved recruitment of PARG-1 to this chromosomal subdomain, similar to SC central elements48,51-54. | ||
| Immunofluorescence analyses showed that PARG-1::GFP is first detected in premeiotic and leptotene/zygotene nuclei and then became progressively enriched along chromosomes throughout pachytene. In late pachytene, PARG-1::GFP showed retraction toward the short arm of the bivalent (a chromosomal subdomain formed in response to CO formation) which was particularly evident at diplotene. In nuclei at the diakinesis stage, PARG-1::GFP was detectable mostly in the nucleoplasm. Co-staining with axial proteins HTP-1/ HTP-3 and the central SC component SYP-138,39,48 revealed recruitment of PARG-1::GFP onto synapsed chromosomes and confirmed its retraction to the short arm of the bivalent in late pachytene cells, which also harbors the chiasma and the central elements of the SC49,50. | |||
| Type | Genome_editing | ||
| Reporter_gene | |||
| Reference | WBPaper00060401 | ||
| Variation | WBVar02157220 |
