WormBase Tree Display for Expr_pattern: Expr15967
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| Expr15967 | Expression_of | Gene | WBGene00009308 | ||
|---|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00009308 | ||||
| Expression_data | Life_stage | WBls:0000041 | Anatomy_term | WBbt:0007850 | |
| Anatomy_term | WBbt:0007850 | Certain | |||
| Life_stage | WBls:0000041 | ||||
| Subcellular_localization | SPE-18 protein localizes to FBs of developing spermatocytes. Within wild-type male gonads, SPE-18 labeling was first detectable in late pachytene spermatocytes and then increased in intensity through the karyosome stage Within developing spermatocytes, SPE-18 labeled numerous discrete structures the pattern and distribution of which seemed similar to FBs. SPE-18 labeling then decreased in intensity through the meiotic divisions and became undetectable in haploid spermatids. This failure to detect SPE18 in haploid sperm was consistent with the absence of a SPE-18 signal in western blots of adult spermatozoa-containing hermaphrodites. | ||||
| Type | Western | ||||
| Pattern | We tested hermaphrodite samples from specific larval stages and found that the major band detected in adult males could only be detected in fourth stage larvae (L4), the only stage when hermaphrodites are actively producing sperm. The notable absence of SPE-18 in adult hermaphrodites that have spermatozoa in their spermathecas suggested that SPE-18 might function in developing and/or meiotically dividing spermatocytes but not in haploid sperm. | ||||
| Reference | WBPaper00061034 | ||||
| Antibody_info | WBAntibody00002931 |
