WormBase Tree Display for Expr_pattern: Expr15986
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Expr15986 | Expression_of | Gene | WBGene00000900 |
---|---|---|---|
Reflects_endogenous_expression_of | WBGene00000900 | ||
Expression_data (2) | |||
Type | Genome_editing | ||
Reporter_gene | |||
Pattern | We found that DAF-4::GFP::3xFLAG is expressed in multiple tissues, including the pharynx, hypodermis, intestine, and developing vulva. Within each cell, DAF-4::GFP::3xFLAG is primarily localized to the cell surface. We also detected some GFP signal in intracellular vesicles in hypodermal cells. Notably, in both the intestine and the vulva, 2 types of polarized epithelial cells, DAF-4::GFP::3xFLAG is localized to the basolateral membrane but absent on the apical membrane. The basolateral localization of DAF-4::GFP::3xFLAG in the intestinal cells has also been previously reported by Gleason and colleagues using a functional, intestinally expressed DAF-4::GFP. | ||
Reference | WBPaper00059222 | ||
Variation | WBVar02157765 |